I think Lancaster should, and will, experiment against Italy. Though he probably won't change as many things as some would like. I think Ashton will stay in, on the proviso that if he doesn't improve his defence, and score, in the next two matches he won't be near the squad for a while. Flood will probably start, given Farrell's injury, maybe with Farrell benched to give 30 minutes in the second half to warm-up. The problem was apparently him getting sufficient kicking practice, so maybe play him and get Flood/Goode/Twelvetrees to kick. Not ideal, but if the idea is to prepare for the Wales game then it'd be better than him getting no rugby whatsoever.
Personally:
1. Marler -- Castrogiovanni will be nice practice for Adam Jones, particularly if he spends all his time binding on the arm.
2. Hartley -- A last chance to see if he improves set play enough to discount the loss of Youngs in the loose.
3. Cole
4. Launchbury
5. Parling
6. Wood
7. Robshaw (c)
8. B. Vunipola -- lets Wood move back to the flank, a relatively easier start to international rugby.
9. Care -- If Flood gets a go, then Care certainly deserves his shot. More likely to play with Flood against Wales than Youngs, so give them time together.
10. Flood
11. Brown -- Would prefer Monye and a move to fullback, to rest/avoid injury, but that's probably not going to happen.
12. Barritt
13. Tuilagi
14. Ashton -- As said, if he doesn't get on shoulders and score tries against Italy, that's the final straw.
15. Goode
16. T. Youngs
17. Wilson -- On at 50. Really doesn't get to play enough, through no fault of his own. Tighthead needs depth built beneath Cole.
18. M. Vunipola -- Needs time; if Marler has a rough game against Wales, then he will be important.
19. Lawes -- Not a blindside, but still had good games against Scotland and Ireland.
20. Haskell -- Solid so far, seems to have reduced his penalty count down. That being said, still managed to get binned against Ireland.
21. B. Youngs -- Half-back rotation.
22. Farrell/Burns -- Former if anywhere near fitness. Burns will get his chance on the Argentina tour, I imagine.
23. Twelvetrees -- Half an hour inside Tuilagi; see if the wingers actually get some ball when both are together.
Obviously I'd prefer a set of young wingers in there, and maybe Burns, but I think that's probably the selection we will see. Half backs given a chance, and Billy gets a go to try and re-balance the back row. Earlier subs to try and give impact players some experience prior to Cardiff. The spine of the team (Cole, Parling, Wood, Robshaw, and Barritt) is intact enough to ensure the experiment shouldn't be too risky.